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anti fpr2 pe (R&D Systems)

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R&D Systems anti fpr2 pe
Anti Fpr2 Pe, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+fpr2+pe/Human+FPR1+APC-conjugated+Antibody/pm37505448-93-59-63
Average 92 stars, based on 3 article reviews

anti fpr2 pe - by Bioz Stars, 2026-09

92/100 stars

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Staining:

Article Title: A Comparative Inflammation‐on‐a‐Chip with a Complete 3D Interface: Pharmacological Applications in COPD‐Induced Neutrophil Migration
Article Snippet: HL‐60 cells and HUVECs were stained with Fixable Viability Dye eflour 780 (1:1000, Thermo Fisher Scientific) for 30 min at 4 °C. .. The cells were then stained with fluorescently conjugated primary antibodies, anti‐CXCR1‐APC (Cat. No. 320 612), anti‐CXCR2‐PE‐Cy7 (Cat. No. 320 716), anti‐CD11b‐BV605 (Cat. No. 301 332), anti‐C5aR‐PerCP‐Cy5 (Cat. No. 344 312), anti‐ICAM‐1 (Cat. No. 353 108), anti‐CD62E (Cat. No. 336 012), anti‐CD62P (Cat. No. 304 920) (BioLegend, San Diego, CA, USA), anti‐LTB4R‐FITC (Cat. No. FAB099F), anti‐FPR1‐APC (Cat. No. FAB3744A), or anti‐FPR2‐PE (Cat. No. FAB3744A) (R&D Systems) in FACS buffer containing 2% (v/v) BSA and 2 mM EDTA in DPBS for 30 min at 4 °C. .. Suspended single cells were washed twice with FACS buffer, and then cells were detected with a BD LSRFortessa TM cell analyzer (BD Biosciences, Franklin Lakes, NJ, USA).

Article Title: A Comparative Inflammation-on-a-Chip with a Complete 3D Interface: Pharmacological Applications in COPD-Induced Neutrophil Migration.
Article Snippet: HL-60 cells and HUVECs were stained with Fixable Viability Dye eflour 780 (1:1000, Thermo Fisher Scientific) for 30 min at 4 °C. .. The cells were then stained with fluorescently conjugated primary antibodies, anti-CXCR1-APC (Cat. No. 320 612), antiCXCR2-PE-Cy7 (Cat. No. 320 716), anti-CD11b-BV605 (Cat. No. 301 332), anti-C5aR-PerCP-Cy5 (Cat. No. 344 312), anti-ICAM-1 (Cat. No. 353 108), anti-CD62E (Cat. No. 336 012), anti-CD62P (Cat. No. 304 920) (BioLegend, San Diego, CA, USA), anti-LTB4RFITC (Cat. No. FAB099F), anti-FPR1-APC (Cat. No. FAB3744A), or anti-FPR2-PE (Cat. No. FAB3744A) (R&D Systems) in FACS buffer containing 2% (v/v) BSA and 2 mM EDTA in DPBS for 30 min at 4 °C. .. Suspended single cells were washed twice with FACS buffer, and then cells were detected with a BD LSRFortessaTM cell analyzer (BD Biosciences, Franklin Lakes, NJ, USA).

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$TNBC cell lines express Annexin A1 (AnxA1). ( A ) The cropped Western blotting of AnxA1 expression in cytoplasm (C), nuclei (N), and supernatants (S) of MCF-10, MCF-7, MDA-MB-231, BT-549, MDA-MB-157 and MDA-MB-453 cells. Full length AnxA1 (37 kDa) and the cleaved AnxA1 form (33 kDa) were detected. β-Actin (42 kDa) and Laminin B2 (72 kDa) were used to check the proper cell fractionation and as loading controls of cytoplasmic and nuclear extracts, respectively. ( B ) ANXA1 mRNA relative level of MCF-10, MCF-7, MDA-MB-231, BT-549, MDA-MB-157 and MDA-MB-453 cells. ( C ) Expression analysis of AnxA1 receptors (FPR1 and FPR2) on the cell surface of MCF-10, MCF-7, MDA-MB-231, BT-549, MDA-MB-157 and MDA-MB-453 cells by flow cytometry (black peak with tracing line). As the negative control, the secondary antibody alone was used (solid gray peak). Three independent experiments were performed. * p < 0.05; ** p < 0.001.$

Journal: Cells

Article Title: Interleukin-6 Signaling in Triple Negative Breast Cancer Cells Elicits the Annexin A1/Formyl Peptide Receptor 1 Axis and Affects the Tumor Microenvironment

doi: 10.3390/cells11101705

Figure Lengend Snippet: TNBC cell lines express Annexin A1 (AnxA1). ( A ) The cropped Western blotting of AnxA1 expression in cytoplasm (C), nuclei (N), and supernatants (S) of MCF-10, MCF-7, MDA-MB-231, BT-549, MDA-MB-157 and MDA-MB-453 cells. Full length AnxA1 (37 kDa) and the cleaved AnxA1 form (33 kDa) were detected. β-Actin (42 kDa) and Laminin B2 (72 kDa) were used to check the proper cell fractionation and as loading controls of cytoplasmic and nuclear extracts, respectively. ( B ) ANXA1 mRNA relative level of MCF-10, MCF-7, MDA-MB-231, BT-549, MDA-MB-157 and MDA-MB-453 cells. ( C ) Expression analysis of AnxA1 receptors (FPR1 and FPR2) on the cell surface of MCF-10, MCF-7, MDA-MB-231, BT-549, MDA-MB-157 and MDA-MB-453 cells by flow cytometry (black peak with tracing line). As the negative control, the secondary antibody alone was used (solid gray peak). Three independent experiments were performed. * p < 0.05; ** p < 0.001.

Article Snippet: The isotype controls for FPR1 ((anti-mouse IgG2A-FITC control) 1:50; IC003F; R&D Systems) and FPR2 ((anti-mouse IgG2B-PE control) 1:50; IC0041P, R&D Systems) were also used.

Techniques: Western Blot, Expressing, Cell Fractionation, Flow Cytometry, Negative Control